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To obtain stable transfectants, cells seeded in six-well plates were transfected with 2.5 μg/well plasmids using 10 μl/well Lipofectamine®LTX Reagent and 2.5 μl/well Plus Reagent. After 6 h incubation in serum and antibiotic free condition, the medium was replaced with RPMI 1640 containing 10% FBS, and the cells were cultured for 48 h before submitting to a 2-week selection in medium containing G418 (600 μg/ml).
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The default installation package for Intel® DAAL for Windows now consists of a smaller installation package that dynamically downloads and then installs selected packages. This requires a working internet connection and potentially a proxy setting if you are behind an internet proxy. Full packages are provided alongside this online install package. You can download them if a working internet connection is not available.
The default installation package for Intel® DAAL for Linux consists of a smaller installation package that dynamically downloads and then installs packages selected to be installed. This requires a working internet connection and potentially a proxy setting if you are behind an internet proxy. Full packages are provided alongside where you download this online install package if a working internet connection is not available.
There are several different product suites available, for example, Intel® Data Analytics Acceleration Library for macOS*, Intel® Parallel Studio XE Composer Edition for C++ macOS*, each including Intel DAAL as one of components. Please read the download web page carefully to determine which product is appropriate for you.
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Chiral α,β-unsaturated acylammonium salts are novel dienophiles enabling enantioselective Diels-Alder-lactonization (DAL) organocascades leading to cis- and trans-fused, bicyclic γ- and δ-lactones from readily prepared dienes, commodity acid chlorides, and a chiral isothiourea organocatalyst under mild conditions. We describe extensions of stereodivergent DAL organocascades to other racemic dienes bearing pendant secondary and tertiary alcohols, and application to a formal synthesis of (+)-dihydrocompactin is described. A combined experimental and computational investigation of unsaturated acylammonium salt formation and the entire DAL organocascade pathway provide a rationalization of the effect of Brønsted base additives and enabled a controllable, diastereodivergent DAL process leading to a full complement of possible stereoisomeric products. Evaluation of free energy and enthalpy barriers in conjunction with experimentally observed temperature effects revealed that the DAL is a rare case of an entropy-controlled diastereoselective process. NMR analysis of diene alcohol-Brønsted base interactions and computational studies provide a plausible explanation of observed stabilization of exo transition-state structures through hydrogen-bonding effects. 2b1af7f3a8