Melissa Taylor, MA, is the creator of Imagination Soup. She's a mother, teacher, author, and freelance writer. She writes Imagination Soup and freelances for publications online and in print, including Sylvan Learning, Random House, USA Today Health, The Writer, and Scholastic Parent and Child.
To obtain stable transfectants, cells seeded in six-well plates were transfected with 2.5 μg/well plasmids using 10 μl/well Lipofectamine®LTX Reagent and 2.5 μl/well Plus Reagent. After 6 h incubation in serum and antibiotic free condition, the medium was replaced with RPMI 1640 containing 10% FBS, and the cells were cultured for 48 h before submitting to a 2-week selection in medium containing G418 (600 μg/ml).
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The default installation package for Intel® DAAL for Windows now consists of a smaller installation package that dynamically downloads and then installs selected packages. This requires a working internet connection and potentially a proxy setting if you are behind an internet proxy. Full packages are provided alongside this online install package. You can download them if a working internet connection is not available.
The default installation package for Intel® DAAL for Linux consists of a smaller installation package that dynamically downloads and then installs packages selected to be installed. This requires a working internet connection and potentially a proxy setting if you are behind an internet proxy. Full packages are provided alongside where you download this online install package if a working internet connection is not available.
There are several different product suites available, for example, Intel® Data Analytics Acceleration Library for macOS*, Intel® Parallel Studio XE Composer Edition for C++ macOS*, each including Intel DAAL as one of components. Please read the download web page carefully to determine which product is appropriate for you.
Learn about over 1,000 camps and ghettos in Volumes I-III of this encyclopedia, which are available as a free PDF download. This reference provides text, photographs, charts, maps, and extensive indexes.
Chiral α,β-unsaturated acylammonium salts are novel dienophiles enabling enantioselective Diels-Alder-lactonization (DAL) organocascades leading to cis- and trans-fused, bicyclic γ- and δ-lactones from readily prepared dienes, commodity acid chlorides, and a chiral isothiourea organocatalyst under mild conditions. We describe extensions of stereodivergent DAL organocascades to other racemic dienes bearing pendant secondary and tertiary alcohols, and application to a formal synthesis of (+)-dihydrocompactin is described. A combined experimental and computational investigation of unsaturated acylammonium salt formation and the entire DAL organocascade pathway provide a rationalization of the effect of Brønsted base additives and enabled a controllable, diastereodivergent DAL process leading to a full complement of possible stereoisomeric products. Evaluation of free energy and enthalpy barriers in conjunction with experimentally observed temperature effects revealed that the DAL is a rare case of an entropy-controlled diastereoselective process. NMR analysis of diene alcohol-Brønsted base interactions and computational studies provide a plausible explanation of observed stabilization of exo transition-state structures through hydrogen-bonding effects. 2b1af7f3a8